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rpoC [2019-07-19 11:27:49]
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rpoC [2019-07-19 11:27:49]

[SW|RNA polymerase] beta' subunit
locus
BSU_01080
pI
8.86
mw
133.92 kDa
protein length
1199 aa Sequence Blast
gene length
3600 bp Sequence Blast
function
[SW|transcription]
product
[SW|RNA polymerase] beta' subunit
essential
yes
ec
2.7.7.6
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.1|RNA polymerase]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • Gene

    Coordinates
    125,562 → 129,161

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Nucleoside triphosphate + RNA(n) --> diphosphate + RNA(n + 1) (according to UniProt)

    • Protein family

  • [SW|RNA polymerase] beta' chain family (single member, according to UniProt)

    • Modification

  • phosphorylated on Arg-335 and Arg-803 [Pubmed|22517742]

    • Structure

  • [PDB|3IYD] ([SW|RNA polymerase] from ''E. coli'', 50% identity) [Pubmed|19903881]

    • [SW|Localization]

  • colocalizes with the nucleoid [Pubmed|25092033]
  • membrane associated [Pubmed|18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB]-[gene|BC8B37355E6FADBA5F4636C3879E99003B0F3E84|rpoC]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7657605], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

    • regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • the leader mRNA is processed upstream of [gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
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    Biological materials

    Expression vector

  • pGP2180: expression of ''rpoC''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

    • References

    Reviews

  • 22210308,24763425,28657884

    • Original publications

  • 7657605,12486038,10675340,7592585,19735077,18763711,19680289,22517742,23070162,20817769,24572018,25092033,24491578,26243774,21357417,26293966,26742846,19903881,17917675,26098117,28507241,28539362,28598017